Angiopoietin-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15385
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Canine (92%), Porcine (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Angiopoietin-2. The exact sequence is proprietary.
Reactivity Notes
Rat (86%), Bovine (89%), Chicken (85%).
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Angiopoietin-2 Antibody
![Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody [NBP2-15385]](https://resources.bio-techne.com/images/products/Angiopoietin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15385-img0001.jpg "Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody [NBP2-15385]")
Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody [NBP2-15385]
Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody [NBP2-15385] - Human colon carcinoma, using Angiopoietin 2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: Angiopoietin-2 Antibody [NBP2-15385]](https://resources.bio-techne.com/images/products/Angiopoietin-2-Antibody-Western-Blot-NBP2-15385-img0003.jpg "Western Blot: Angiopoietin-2 Antibody [NBP2-15385]")
Western Blot: Angiopoietin-2 Antibody [NBP2-15385]
Western Blot: Angiopoietin-2 Antibody [NBP2-15385] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Angiopoietin-2 Antibody [NBP2-15385] -
Western Blot: Angiopoietin-2 Antibody [NBP2-15385] - HepG2 conditioned medium (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.Applications for Angiopoietin-2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Angiopoietin-2
Alternate Names
ANGPT2
Gene Symbol
ANGPT2
Additional Angiopoietin-2 Products
Product Specific Notices for Angiopoietin-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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