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Angiopoietin-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32607

Novus Biologicals, part of Bio-Techne
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NBP1-32607

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Angiopoietin-4. The exact sequence is proprietary.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Angiopoietin-4 Antibody

Western Blot: Angiopoietin-4 Antibody [NBP1-32607]

Western Blot: Angiopoietin-4 Antibody [NBP1-32607]

Western Blot: Angiopoietin-4 Antibody [NBP1-32607] - Sample (30ug whole cell lysate)A:H1299 B:Hep G2 C:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607]

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607]

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607] - Paraffin-embedded mouse duodenum. Angiopoietin 4 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607]

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607]

Immunohistochemistry-Paraffin: Angiopoietin-4 Antibody [NBP1-32607] - Paraffin-embedded DU145 xenograft, using antibody at 1:100 dilution.

Applications for Angiopoietin-4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Angiopoietin-4

Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.

Alternate Names

ANGPT4

Entrez Gene IDs

51378 (Human)

Gene Symbol

ANGPT4

UniProt

Additional Angiopoietin-4 Products

Product Documents for Angiopoietin-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Angiopoietin-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.