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Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60244

Novus Biologicals, part of Bio-Techne
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NBP2-60244-100ul
NBP2-60244-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 23D8

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free

Immunogen

Recombinant human Angiopoietin-like Protein 3/ANGPTL3 (243-460aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8)BSA Free [NBP2-60244]

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8)BSA Free [NBP2-60244]

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) [NBP2-60244] - The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Angiopoietin-like Protein 3/ANGPTL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse kidney tissue lysate
Immunocytochemistry/ Immunofluorescence: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free [NBP2-60244]

Immunocytochemistry/ Immunofluorescence: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free [NBP2-60244]

Immunocytochemistry/Immunofluorescence: Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) [NBP2-60244] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Applications for Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a 460 amino acid protein that is found most commonly in the liver, but has also been shown to reside in kidney samples, and contains three domains consisting of a signal peptide, N-terminal coiled-coil and C-terminal Fibrinogen (FBN) like domain. This protein is involved with the lipid metabolism disease pathways in relation to low levels of LDL (low density lipoproteins), HDL (High density lipoproteins), and triglycerides in plasma. This protein plays a role in angiogenesis and has been shown to interact with ITGAV, ITGB3, and ITGA5, and work is being done to study the protein and the related hypobetalipoproteinemia and atherosclerosis involvement.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Gene Symbol

ANGPTL3

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Angiopoietin-like Protein 3/ANGPTL3 Antibody (23D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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