Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7)

Novus Biologicals, part of Bio-Techne | Catalog # H00027329-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Angiopoietin-like Protein 3/ANGPTL3 Antibodies »

Product Specifications

Immunogen

ANGPTL3 (AAH58287, 17 a.a. ~ 460 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SRIDQDNSSFDSLSPEPKSRFAMLDDVKILANGLLQLGHGLKDFVHKTKGQINDIFQKLNIFDQSFYDLSLQTSEIKEEEKELRRTTYKLQVKNEEVKNMSLELNSKLESLLEEKILLQQKVKYLEEQLTNLIQNQPETPEHPEVTSLKTFVEKQDNSIKDLLQTVEDQYKQLNQQHSQIKEIENQLRRTSIQEPTEISLSSKPRAPRTTPFLQLNEIRNVKHDGIPAECTTIYNRGEHTSGMYAIRPSNSQVFHVYCDVISGSPWTLIQHRIDGSQNFNETWENYKYGFGRLDGEFWLGLEKIYSIVKQSNYVLRIELEDWKDNKHYIEYSFYLGNHETNYTLHLVAITGNVPNAIPENKDLVFSTWDHKAKGHFNCPEGYSGGWWWHDECGENNLNGKYNKPRAKSKPERRRGLSWKSQNGRLYSIKSTKMLIHPTDSESFE

Specificity

ANGPTL3 - angiopoietin-like 3 (3B7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7)

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01]

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01]

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01] - Analysis of ANGPTL3 expression in transfected 293T cell line by ANGPTL3 monoclonal antibody (M01), clone 3B7.Lane 1: ANGPTL3 transfected lysate (Predicted MW: 53.6 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01]

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01]

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01] - Analysis of monoclonal antibody to ANGPTL3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
ELISA: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01]

ELISA: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01]

ELISA: Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7) [H00027329-M01] - Detection limit for recombinant GST tagged ANGPTL3 is 1 ng/ml as a capture antibody.

Applications for Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Angiopoietin-like Protein 3/ANGPTL3

The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. [provided by RefSeq]

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human)

Gene Symbol

ANGPTL3

UniProt

AAH58287 (Human)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Angiopoietin-like Protein 3/ANGPTL3 Antibody (3B7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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