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ANKRD23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90599

Novus Biologicals, part of Bio-Techne
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NBP1-90599
NBP1-90599-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KRLRHRVPPRKPEPLVKPQSQAQVEPVGLEMFLKAAAENQEYLIDKYLTDGGDPNAHDKLHRTALHWAC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD23 Antibody

Western Blot: ANKRD23 Antibody [NBP1-90599]

Western Blot: ANKRD23 Antibody [NBP1-90599]

Western Blot: ANKRD23 Antibody [NBP1-90599] - Analysis in control (vector only transfected HEK293T lysate) and ANKRD23 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ANKRD23 Antibody [NBP1-90599]

Immunocytochemistry/ Immunofluorescence: ANKRD23 Antibody [NBP1-90599]

Immunocytochemistry/Immunofluorescence: ANKRD23 Antibody [NBP1-90599] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ANKRD23 Antibody [NBP1-90599]

Immunohistochemistry-Paraffin: ANKRD23 Antibody [NBP1-90599]

Immunohistochemistry-Paraffin: ANKRD23 Antibody [NBP1-90599] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for ANKRD23 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANKRD23

Alternate Names

ankyrin repeat domain 23, DARP, diabetes-related ankyrin repeat protein, MARP3

Entrez Gene IDs

200539 (Human)

Gene Symbol

ANKRD23

UniProt

Additional ANKRD23 Products

Product Documents for ANKRD23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ANKRD23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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