ANKZF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84627
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YEEDPREAVRLHSPQTHWKTVREERKKPTEEEIRKICRDEKEALGQNEESPKQGSGSEGEDGFQVELELVELTVGTLDLCESEVLPKR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ANKZF1 Antibody
Western Blot: ANKZF1 Antibody [NBP1-84627]
Western Blot: ANKZF1 Antibody [NBP1-84627] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG spImmunocytochemistry/ Immunofluorescence: ANKZF1 Antibody [NBP1-84627]
Immunocytochemistry/Immunofluorescence: ANKZF1 Antibody [NBP1-84627] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: ANKZF1 Antibody [NBP1-84627]
Immunohistochemistry-Paraffin: ANKZF1 Antibody [NBP1-84627] - Staining shows positivity in smooth muscle and endothelial cells.Applications for ANKZF1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ANKZF1
Alternate Names
ankyrin repeat and zinc finger domain containing 1, ankyrin repeat and zinc finger domain-containing protein 1, FLJ13144, Zinc finger protein 744, ZNF744FLJ10415
Entrez Gene IDs
55139 (Human)
Gene Symbol
ANKZF1
UniProt
Additional ANKZF1 Products
Product Documents for ANKZF1 Antibody
Product Specific Notices for ANKZF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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