Annexin A2 Antibody (1G7)

Novus Biologicals, part of Bio-Techne | Catalog # H00000302-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Annexin A2 Antibodies »

Product Specifications

Immunogen

ANXA2 (AAH66955, 19 a.a. ~ 357 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISVMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28652618).

Specificity

ANXA2 - annexin A2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Annexin A2 Antibody (1G7)

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Localization of annexin A2 at the focal adhesions: KS1767 cells co-stained for paxillin (FITC-conjugated secondary antibody, green) and annexin A2 (Alexa 647-conjugated secondary antibody, red). An enlarged inset (white dotted line) is included for prompt visualization. Nuclei are visualized with DAPI (blue); scale bar, 10um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Co-localization analysis: KS1767 cells grown overnight onto circular coverslips were incubated for 2h at 37degrees C with biotinylated LGRFYAASG-pen, or unconjugated LGRFYAASG (10uM). Untreated cells were used as controls. IF staining of biotinylated peptides (avidin-FITC, green) and annexin A2 (Cy3-conjugated secondary antibody, red) was analyzed with Fiji ImageJ. Co-localized pixels are visualized in white (arrows), and Manders' overlap coefficients are reported in the graph (**P<0.01; ***P<0.001 versus LGRFYAASG-pen). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.
Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. ANXA2 Antibody (H00000302-M02) was used for primary antibody: 1:2000, 4C, overnight. WB image submitted by a verified customer review.
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Applications for Annexin A2 Antibody (1G7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using H00000302-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Annexin A2

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV

Entrez Gene IDs

302 (Human)

Gene Symbol

ANXA2

OMIM

151740 (Human)

UniProt

AAH66955 (Human)

Additional Annexin A2 Products

Product Documents for Annexin A2 Antibody (1G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Annexin A2 Antibody (1G7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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