Annexin A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-881

Novus Biologicals, part of Bio-Techne
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NB100-881
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Bovine

Predicted:

Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Annexin A2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence STVHEILCKLSLEGD corresponding to N-Terminus according to NP_004030.1.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25827591).

Specificity

This antibody is expected to recognise both reported isoforms (NP_001002858.1; NP_004030.1). Variants NP_004030.1, NP_001002857.1 and NP_001129487.1 represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Annexin A2 Antibody

Western Blot: Annexin A2 Antibody [NB100-881]

Western Blot: Annexin A2 Antibody [NB100-881]

Western Blot: Annexin A2 Antibody [NB100-881] - Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for Annexin A2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10 - 1:500

Peptide ELISA

Detection limit 1:1000

Western Blot

0.5 - 3 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in A549, HeLa and NIH3T3 lysates (calculated MW of 38.6 kDa band according to NP_004030.1 and NP_001002857.1). IHC-P usage is reported in scientific literature (PMID: 25827591).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Annexin A2

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV

Entrez Gene IDs

302 (Human); 12306 (Mouse); 56611 (Rat)

Gene Symbol

ANXA2

UniProt

P07355 (Human)

Additional Annexin A2 Products

Product Documents for Annexin A2 Antibody

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Product Specific Notices for Annexin A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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