Annexin A3 Antibody (OTI1A5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70179
![Western Blot: Annexin A3 Antibody (OTI1A5)Azide and BSA Free [NBP2-70179]](https://resources.bio-techne.com/images/products/Annexin-A3-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-70179-img0001.jpg "Western Blot: Annexin A3 Antibody (OTI1A5)Azide and BSA Free [NBP2-70179]")
![Western Blot: Annexin A3 Antibody (OTI1A5)Azide and BSA Free [NBP2-70179]](https://resources.bio-techne.com/images/products/Annexin-A3-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-70179-img0002.jpg "Western Blot: Annexin A3 Antibody (OTI1A5)Azide and BSA Free [NBP2-70179]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1A5
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human ANXA3 (NP_005130) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
36.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Annexin A3 Antibody (OTI1A5) - Azide and BSA Free
Western Blot: Annexin A3 Antibody (OTI1A5)Azide and BSA Free [NBP2-70179]
Western Blot: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Annexin A3.![Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]](https://resources.bio-techne.com/images/products/Annexin-A3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70179-img0005.jpg "Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]")
Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]
Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179] - Staining of paraffin-embedded Human lung tissue using anti-Annexin A3 mouse monoclonal antibody.![Flow Cytometry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]](https://resources.bio-techne.com/images/products/Annexin-A3-Antibody-OTI1A5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70179-img0003.jpg "Flow Cytometry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]")
Flow Cytometry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]
Flow Cytometry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Annexin A3 antibody, and then analyzed by flow cytometry.![Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]](https://resources.bio-techne.com/images/products/Annexin-A3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70179-img0004.jpg "Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]")
![Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]](https://resources.bio-techne.com/images/products/Annexin-A3-Antibody-OTI1A5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70179-img0006.jpg "Immunohistochemistry: Annexin A3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-70179]")
Applications for Annexin A3 Antibody (OTI1A5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Annexin A3
Alternate Names
ANXA3, Calcimedin 35-alpha, Lipocortin III, PAP-III
Gene Symbol
ANXA3
Additional Annexin A3 Products
Product Documents for Annexin A3 Antibody (OTI1A5) - Azide and BSA Free
Product Specific Notices for Annexin A3 Antibody (OTI1A5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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