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Annexin A4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90151

Novus Biologicals, part of Bio-Techne
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NBP1-90151-25ul
NBP1-90151

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KGGTVKAASGFNAMEDAQTLRKAMKGLGTDEDAIISVLAYRNTAQRQEIRTAYKSTIGRDLIDDLKSELSGNFEQVIVGMMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRISQTYQQQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Annexin A4 Antibody

Western Blot: Annexin A4 Antibody [NBP1-90151]

Western Blot: Annexin A4 Antibody [NBP1-90151]

Western Blot: Annexin A4 Antibody [NBP1-90151] - Analysis in human cell lines A-549 and HeLa using anti-ANXA4 antibody. Corresponding ANXA4 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Immunocytochemistry/ Immunofluorescence: Annexin A4 Antibody [NBP1-90151]

Immunocytochemistry/ Immunofluorescence: Annexin A4 Antibody [NBP1-90151]

Immunocytochemistry/Immunofluorescence: Annexin A4 Antibody [NBP1-90151] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: Annexin A4 Antibody [NBP1-90151]

Immunohistochemistry-Paraffin: Annexin A4 Antibody [NBP1-90151]

Immunohistochemistry-Paraffin: Annexin A4 Antibody [NBP1-90151] - Staining of human pancreas shows distinct positivity in ductal cells.

Applications for Annexin A4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin A4

Annexin A4, also known as Annexin A4, belongs to the annexin family of calcium dependent phospholipid binding proteins. Annexin A4 is almost exclusively expressed in epithelial cells.

Alternate Names

AIV, Annexin IV, ANX4, ANXA4, Lipocortin IV, PIG28, ZAP36

Entrez Gene IDs

307 (Human)

Gene Symbol

ANXA4

UniProt

Additional Annexin A4 Products

Product Documents for Annexin A4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Annexin A4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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