Annexin V Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38248

Novus Biologicals, part of Bio-Techne
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NBP2-38248
NBP2-38248-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Rat

Predicted:

Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Annexin V Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Annexin V Antibody

Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]

Immunocytochemistry/ Immunofluorescence: Annexin V Antibody [NBP2-38248]

Immunocytochemistry/Immunofluorescence: Annexin V Antibody [NBP2-38248] - Staining of human cell line PC-3 shows localization to nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]

Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]

Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248] - Staining of human prostate shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]

Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248]

Immunohistochemistry-Paraffin: Annexin V Antibody [NBP2-38248] - Analysis in human placenta and skeletal muscle tissues. Corresponding ANXA5 RNA-seq data are presented for the same tissues.
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Applications for Annexin V Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin V

Annexin V is encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Alternate Names

ANX5, ANXA5, ANXV

Gene Symbol

ANXA5

UniProt

P08758

Additional Annexin V Products

Product Documents for Annexin V Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Annexin V Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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