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ANP32A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49519

Novus Biologicals, part of Bio-Techne
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NBP2-49519
NBP2-49519-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GLEVLAEKCPNLTHLNLSGNKIKDLSTIEPLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANP32A Antibody

Western Blot: ANP32A Antibody [NBP2-49519]

Western Blot: ANP32A Antibody [NBP2-49519]

Western Blot: ANP32A Antibody [NBP2-49519] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: ANP32A Antibody [NBP2-49519]

Immunocytochemistry/ Immunofluorescence: ANP32A Antibody [NBP2-49519]

Immunocytochemistry/Immunofluorescence: ANP32A Antibody [NBP2-49519] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & centrosome.
Immunohistochemistry: ANP32A Antibody [NBP2-49519]

Immunohistochemistry: ANP32A Antibody [NBP2-49519]

Immunohistochemistry: ANP32A Antibody [NBP2-49519] - Staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.

Applications for ANP32A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANP32A

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression

Alternate Names

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A, acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, cerebellar leucine rich acidic nuclear protein, I1PP2A, inhibitor-1 of protein phosphatase-2A, LANPPutative HLA-DR-associated protein I, Leucine-rich acidic nuclear protein, MAPMC15orf1, mapmodulin, PHAP1MGC150373, PHAPIMGC119787, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, PP32, putative human HLA class II associated protein I

Gene Symbol

ANP32A

Additional ANP32A Products

Product Documents for ANP32A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ANP32A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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