ANP32A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81794

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ANP32A Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ANP32A . The immunogen is located within the last 50 amino acids of ANP32A. Amino Acid Squence: KRKREPEDEGEDDD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%)

Specificity

This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANP32A Antibody - BSA Free

Western Blot: ANP32A AntibodyBSA Free [NBP2-81794]

Western Blot: ANP32A AntibodyBSA Free [NBP2-81794]

Western Blot: ANP32A Antibody [NBP2-81794] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: ANP32A NBP2-81794 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: ANP32A Antibody - BSA Free [NBP2-81794]

Immunocytochemistry/ Immunofluorescence: ANP32A Antibody - BSA Free [NBP2-81794]

Immunocytochemistry/Immunofluorescence: ANP32A Antibody [NBP2-81794] - Immunofluorescence Validation of ANP32A in Mouse Small Intestine cells. Immunofluorescent analysis of 4% paraformaldehydefixed Mouse Small Intestine Cells labeling ANP32A with NBP2-81794 at 20 ug/mL, followed by goat anti-rabbit IgGsecondary antibody at 1/500 dilution (red).
Immunohistochemistry: ANP32A Antibody - BSA Free [NBP2-81794]

Immunohistochemistry: ANP32A Antibody - BSA Free [NBP2-81794]

Immunohistochemistry: ANP32A Antibody [NBP2-81794] - Immunohistochemistry Validation of ANP32A in Mouse Small Intestine TissueImmunohistochemical analysis of paraffin-embedded Mouse Small Intestine Tissue using anti-ANP32A antibody [NBP2-81794] at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrievalwas by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Applications for ANP32A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2 ug/mL

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANP32A

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression

Alternate Names

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A, acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, cerebellar leucine rich acidic nuclear protein, I1PP2A, inhibitor-1 of protein phosphatase-2A, LANPPutative HLA-DR-associated protein I, Leucine-rich acidic nuclear protein, MAPMC15orf1, mapmodulin, PHAP1MGC150373, PHAPIMGC119787, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, PP32, putative human HLA class II associated protein I

Gene Symbol

ANP32A

Additional ANP32A Products

Product Documents for ANP32A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ANP32A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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