ANP32B Antibody (4A8E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16199

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16199-100ul
NBP3-16199-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4A8E5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ANP32B Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ANP32B (Q92688). MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANP32B Antibody (4A8E5)

Western Blot: ANP32B Antibody (4A8E5) [NBP3-16199]

Western Blot: ANP32B Antibody (4A8E5) [NBP3-16199]

Western Blot: ANP32B Antibody (4A8E5) [NBP3-16199] - Western blot analysis of extracts of various cell lines, using ANP32B Rabbit mAb (NBP3-16199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (4A8E5) [NBP3-16199]

Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (4A8E5) [NBP3-16199]

Immunocytochemistry/Immunofluorescence: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunofluorescence analysis of NIH-3T3 cells using ANP32B Rabbit mAb (NBP3-16199) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ANP32B Antibody (4A8E5) [NBP3-16199]

Immunohistochemistry-Paraffin: ANP32B Antibody (4A8E5) [NBP3-16199]

Immunohistochemistry-Paraffin: ANP32B Antibody (4A8E5) [NBP3-16199] - Immunohistochemistry of paraffin-embedded mouse kidney using ANP32B Rabbit mAb (NBP3-16199) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for ANP32B Antibody (4A8E5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ANP32B

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity

Alternate Names

acidic (leucine-rich) nuclear phosphoprotein 32 family, member B, acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, APRILSilver-stainable protein SSP29, Pal31, PHAPI2SSP29, Putative HLA-DR-associated protein I-2

Gene Symbol

ANP32B

Additional ANP32B Products

Product Documents for ANP32B Antibody (4A8E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ANP32B Antibody (4A8E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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