ANP32B Antibody (8E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00010541-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ANP32B Antibodies »

Product Specifications

Immunogen

ANP32B (NP_006392.1, 1 a.a. ~ 251 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD

Specificity

This product is specific for Human ANP32B monoclonal antibody (M05), clone 8E10 [Gene ID: 10541].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ANP32B Antibody (8E10)

Western Blot: ANP32B Antibody (8E10) [H00010541-M05]

Western Blot: ANP32B Antibody (8E10) [H00010541-M05]

Western Blot: ANP32B Antibody (8E10) [H00010541-M05] - Analysis of ANP32B expression in transfected 293T cell line by ANP32B monoclonal antibody (M05), clone 8E10. Lane 1: ANP32B transfected lysate (Predicted MW: 28.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (8E10) [H00010541-M05]

Immunocytochemistry/ Immunofluorescence: ANP32B Antibody (8E10) [H00010541-M05]

Immunocytochemistry/Immunofluorescence: ANP32B Antibody (8E10) [H00010541-M05] - Analysis of monoclonal antibody to ANP32B on HeLa cell. Antibody concentration 10 ug/ml
ELISA: ANP32B Antibody (8E10) [H00010541-M05]

ELISA: ANP32B Antibody (8E10) [H00010541-M05]

ELISA: ANP32B Antibody (8E10) [H00010541-M05] - Detection limit for recombinant GST tagged ANP32B is 0.3 ng/ml as a capture antibody.

Applications for ANP32B Antibody (8E10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Mouse monoclonal antibody raised against a full-length recombinant ANP32B.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ANP32B

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity

Alternate Names

acidic (leucine-rich) nuclear phosphoprotein 32 family, member B, acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, APRILSilver-stainable protein SSP29, Pal31, PHAPI2SSP29, Putative HLA-DR-associated protein I-2

Gene Symbol

ANP32B

UniProt

NP_006392.1 (Human)

Additional ANP32B Products

Product Documents for ANP32B Antibody (8E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ANP32B Antibody (8E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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