Anti- Teplizumab (Anti-Idiotype) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10245
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Multi-Species
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2505B
Product Specifications
Immunogen
Full-length Teplizumab
Specificity
Detects Teplizumab in Western blots and direct ELISAs. In Western blots, no cross-reactivity with Daclizumab, Basiliximab, Satumomab, and Alemtuzumab is observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Anti- Teplizumab (Anti-Idiotype) Antibody
Detection of Teplizumab by Western Blot.
Western blot shows Daclizumab (negative control), Basiliximab (negative control), Satumomab (negative control), Teplizumab, and Alemtuzumab (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Anti- Teplizumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10245) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Teplizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Anti-CD3 (Teplizumab) on Human PBMC by flow cytometry.
Human PBMC were stained with (A) Human CD3 (Research Grade Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929)or (B) Rabbit IgG control antibody (Catalog # MAB1050), followed by Rabbit anti-CD3 (Teplizumab) (Anti-Idiotype, Catalog # MAB10245), then APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111), followed by Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.Applications for Anti- Teplizumab (Anti-Idiotype) Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMC stained with Anti- Human CD3 (Teplizumab), Catalog # MAB9929
Sample: Human PBMC stained with Anti- Human CD3 (Teplizumab), Catalog # MAB9929
Western Blot
2 µg/mL
Sample: Teplizumab
under non-reducing conditions only
Sample: Teplizumab
under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Teplizumab
Additional Teplizumab Products
Product Documents for Anti- Teplizumab (Anti-Idiotype) Antibody
Product Specific Notices for Anti- Teplizumab (Anti-Idiotype) Antibody
For research use only
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