Normal Rabbit IgG Control
R&D Systems, part of Bio-Techne | Catalog # MAB1050
Recombinant Monoclonal Isotype Control
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Rabbit
Cited:
Human, Transgenic Mouse
Applications
Validated:
Control, CyTOF-ready
Cited:
Immunocytochemistry, In vivo assay
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 60024B
Product Specifications
Immunogen
KLH
Specificity
Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Normal Rabbit IgG Control
Detection of Rabbit IgG Control by Flow Cytometry
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Normal Rabbit IgG Control
Application
Recommended Usage
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Normal Rabbit IgG Control
Product Specific Notices for Normal Rabbit IgG Control
For research use only
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