AP2M1 Antibody (OTI1E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00834
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Monkey
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1E9
Concentration
0.65 mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1(NP_001020376) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
49.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AP2M1 Antibody (OTI1E9)
Western Blot: AP2M1 Antibody (1E9) [NBP2-00834] Analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (OTI1E9) [NBP2-00834]
Immunocytochemistry/Immunofluorescence: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1.Immunohistochemistry-Paraffin: AP2M1 Antibody (OTI1E9) [NBP2-00834]
Immunohistochemistry-Paraffin: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of paraffin-embedded Human colon tissue using anti-AP2M1 mouse monoclonal antibody.Applications for AP2M1 Antibody (OTI1E9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:200-500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.65 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AP2M1
Alternate Names
Adaptin-mu2, adaptor-related protein complex 2, mu 1 subunit, AP-2 mu chain, Clathrin assembly protein complex 2 medium chain, clathrin coat adaptor protein AP50, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, clathrin-associated/assembly/adaptor protein, medium 1, HA2 50 kDa subunit, KIAA0109, mu subunit, Plasma membrane adaptor AP-2 50 kDa protein, plasma membrane adaptor AP-2 50kDA protein
Entrez Gene IDs
1173 (Human)
Gene Symbol
AP2M1
Additional AP2M1 Products
Product Documents for AP2M1 Antibody (OTI1E9)
Product Specific Notices for AP2M1 Antibody (OTI1E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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