Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 2E12

Format

BSA Free

Concentration

1 mg/ml

View all APAF-1 Antibodies »

Product Summary for APAF-1 Antibody (2E12) - BSA Free

Immunogen

Recombinant human APAF-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Specificity

Recognizes the CARD domain of human APAF-1. Does not cross-react with mouse or rat APAF-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for APAF-1 Antibody (2E12) - BSA Free

Western Blot: APAF-1 Antibody (2E12)BSA Free [NBP2-80092]

Western Blot: APAF-1 Antibody (2E12)BSA Free [NBP2-80092]

Western Blot: APAF-1 Antibody (2E12) [NBP2-80092] - Analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS).
Immunohistochemistry-Paraffin: APAF-1 Antibody (2E12) - BSA Free [NBP2-80092]

Immunohistochemistry-Paraffin: APAF-1 Antibody (2E12) - BSA Free [NBP2-80092]

Immunohistochemistry-Paraffin: APAF-1 Antibody (2E12) [NBP2-80092] - Immunohistochemical staining of Apaf-1 using anti-Apaf-1 (human), mAb (2E12) in formalin-fixed and paraffin-embedded (FFPE) human kidney tissue (10 ug/mL).

Applications for APAF-1 Antibody (2E12) - BSA Free

Application
Recommended Usage

Immunohistochemistry

10 ug/mL

Immunohistochemistry-Paraffin

10 ug/mL

Immunoprecipitation

1:1000 - 1:2000

Western Blot

1:1000 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APAF-1

Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is an approx. 130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001).

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Gene Symbol

APAF1

Additional APAF-1 Products

Product Documents for APAF-1 Antibody (2E12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APAF-1 Antibody (2E12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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