APAF-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89050

Novus Biologicals, part of Bio-Techne
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NBP1-89050-25ul
NBP1-89050
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (95%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all APAF-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDR

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26259033).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APAF-1 Antibody

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050]

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050]

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Analysis in human lymph node and pancreas tissues. Corresponding APAF1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050]

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050]

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Staining of human pancreas shows weak to moderate positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050]

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050]

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
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Applications for APAF-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200
Application Notes
IHC reported in scientific literature (PMID: 26259033). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APAF-1

Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is an approx. 130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001).

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Entrez Gene IDs

317 (Human)

Gene Symbol

APAF1

UniProt

O14727 (Human)

Additional APAF-1 Products

Product Documents for APAF-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for APAF-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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