APC Antibody (5F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26505
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5F2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human APC [UniProt P25054]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for APC Antibody (5F2)
Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] -
Immunohistochemistry: APC Antibody (5F2) [NBP3-26505] - Image of APC Antibody (5F2) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: APC Antibody (5F2) [NBP3-26505] -
Western Blot: APC Antibody (5F2) [NBP3-26505] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate.All lanes: APC Antibody at 1:1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 312, 301, 309 kDa
Observed band size: 160 kDa
Applications for APC Antibody (5F2)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: APC
Long Name
Adenomatosis Polyposis Coli
Alternate Names
DP2, DP3, FPC, GS, Min
Gene Symbol
APC
Additional APC Products
Product Documents for APC Antibody (5F2)
Product Specific Notices for APC Antibody (5F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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