APC Antibody (KT44)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23227
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # KT44
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone KT44 was generated from a recombinant protein containing amino acid residues from the central region mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2. [UniProt# Q61315]
Specificity
This antibody detects full length APC at 300 kDa and proteolytic fragments of APC at lower molecular weights. The antibody detects APC or its fragments in SW480, CaCO2, DLD-1, and Hct116 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Theoretical MW
300 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Applications for APC Antibody (KT44)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:250
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: APC
Long Name
Adenomatosis Polyposis Coli
Alternate Names
DP2, DP3, FPC, GS, Min
Gene Symbol
APC
Additional APC Products
Product Documents for APC Antibody (KT44)
Product Specific Notices for APC Antibody (KT44)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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