Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Apc10 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the center of human APC10. The immunogen is located within amino acids 60 - 110 of APC10. Amino Acid Squence: IYADYKSDESYTPSK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apc10 Antibody - BSA Free

Western Blot: Apc10 AntibodyBSA Free [NBP1-77157]

Western Blot: Apc10 AntibodyBSA Free [NBP1-77157]

Western Blot: Apc10 Antibody [NBP1-77157] - analysis of APC10 in mouse heart tissue lysate with APC10 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry: Apc10 Antibody - BSA Free [NBP1-77157]

Immunohistochemistry: Apc10 Antibody - BSA Free [NBP1-77157]

Immunohistochemistry: Apc10 Antibody [NBP1-77157] - Mouse heart tissue at 20 ug/mL.
Immunohistochemistry-Paraffin: Apc10 Antibody - BSA Free [NBP1-77157]

Immunohistochemistry-Paraffin: Apc10 Antibody - BSA Free [NBP1-77157]

Immunohistochemistry-Paraffin: Apc10 Antibody [NBP1-77157] - Mouse heart tissue with APC10 antibody at 5 ug/mL.
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Applications for Apc10 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apc10

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.

Alternate Names

anaphase promoting complex subunit 10, anaphase-promoting complex subunit 10, APC10DOC1, Cyclosome subunit 10, DKFZP564L0562

Gene Symbol

ANAPC10

UniProt

EAX05052 (Human)

Additional Apc10 Products

Product Documents for Apc10 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Apc10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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