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Apc10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30725

Novus Biologicals, part of Bio-Techne
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NBP3-30725

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Between 1 and 50

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apc10 Antibody

Apc10 Antibody

Immunoprecipitation:Apc10 AntibodyNBP3-30725] -

Immunoprecipitation:Apc10 AntibodyNBP3-30725] -Detection of human ANAPC10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ANAPC10 antibody NBP3-30725 (lot NBP3-30725-1) used for IP at 6 µg per reaction. ANAPC10 was also immunoprecipitated by rabbit anti-ANAPC10.. For blotting immunoprecipitated ANAPC10, NBP3-30725 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Apc10 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Apc10

APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Alternate Names

anaphase promoting complex subunit 10, anaphase-promoting complex subunit 10, APC10DOC1, Cyclosome subunit 10, DKFZP564L0562

Gene Symbol

ANAPC10

Additional Apc10 Products

Product Documents for Apc10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apc10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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