Apc5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55319

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Apc5 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 705 and the C-terminus (residue 755) of human anaphase promoting complex subunit 5 using the numbering given in entry NP_057321.2 (GeneID 51433).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apc5 Antibody

Western Blot: Apc5 Antibody [NB100-55319]

Western Blot: Apc5 Antibody [NB100-55319]

Western Blot: Apc5 Antibody [NB100-55319] - Detection of human APC5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody NB100-55319 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Apc5 Antibody [NB100-55319]

Western Blot: Apc5 Antibody [NB100-55319]

Western Blot: Apc5 Antibody [NB100-55319] - Detection of mouse APC5 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody NB100-55319 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Apc5 Antibody [NB100-55319]

Immunoprecipitation: Apc5 Antibody [NB100-55319]

Immunoprecipitation: Apc5 Antibody [NB100-55319] - Detection of human APC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC5 antibody NB100-55319 (lot NB100-55319-2) used for IP at 3 ug per reaction. APC5 was also immunoprecipitated by a previous lot of this antibody (lot NB100-55319-1) and another rabbit anti-APC5 antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Apc5 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:500-1:2500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Apc5

APC5 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. APC5 may serve a scaffolding role that connects the enzymatic core APC subunits with the regulatory APC subunits.

Alternate Names

anaphase promoting complex subunit 5, anaphase-promoting complex subunit 5, APC5Cyclosome subunit 5

Entrez Gene IDs

51433 (Human); 59008 (Mouse)

Gene Symbol

ANAPC5

UniProt

NP_057321.2 (Human)

Additional Apc5 Products

Product Documents for Apc5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Apc5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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