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Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07203

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # APOA4/3372

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Human recombinant Apolipoprotein A-IV/ApoA4 protein fragment (around aa 239-371) (exact sequence is proprietary) (Uniprot: P06727)

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08947)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203]

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203] - Formalin-fixed, paraffin-embedded human liver stained with Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody (APOA4/3372).
Protein Array: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203]

Protein Array: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203]

Protein Array: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody (APOA4/3372).
Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203] -

Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372) [NBP3-07203] - Formalin-fixed, paraffin-embedded human liver carcinoma stained with Apolipoprotein A-IV/ApoA4 Mouse Monoclonal Antibody (APOA4/3372).

Applications for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Apolipoprotein A-IV/ApoA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Alternate Names

Apolipoprotein AIV

Gene Symbol

APOA4

UniProt

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein A-IV/ApoA4 Antibody (APOA4/3372)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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