I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?

Name: Apolipoprotein A-IV/ApoA4 Antibody
Brand: Novus Biologicals
SKU: NB100-57089

All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Apolipoprotein A-IV/ApoA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57089

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Apolipoprotein A-IV/ApoA4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KEKESQDKTLSLP corresponding to internal region (near C-Terminus) according to NP_000473.2.

Epitope

KEKESQDKTLSLP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Apolipoprotein A-IV/ApoA4 Antibody

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NB100-57089]

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NB100-57089] - Staining of Human Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for Apolipoprotein A-IV/ApoA4 Antibody

Application

Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01 - 0.03 ug/ml

Application Notes

Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 45.4kDa according to NP_000473.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein A-IV/ApoA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Alternate Names

Apolipoprotein AIV

Entrez Gene IDs

337 (Human)

Gene Symbol

APOA4

UniProt

NP_000473.2 (Human)

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Apolipoprotein A-IV/ApoA4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Apolipoprotein A-IV/ApoA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Apolipoprotein A-IV/ApoA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57089

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Apolipoprotein A-IV/ApoA4 Antibodies »

Product Specifications

Immunogen

Epitope

Clonality

Host

Isotype

Scientific Data Images for Apolipoprotein A-IV/ApoA4 Antibody

Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NB100-57089]

Applications for Apolipoprotein A-IV/ApoA4 Antibody

Peptide ELISA

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Apolipoprotein A-IV/ApoA4

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Apolipoprotein A-IV/ApoA4 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Apolipoprotein A-IV/ApoA4 Antibody