Apolipoprotein A5 Antibody (1G5G9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55454

Novus Biologicals, part of Bio-Techne
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NB110-55454
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G5G9

Format

BSA Free

Concentration

1.9 mg/ml

View all Apolipoprotein A5 Antibodies »

Product Specifications

Immunogen

Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein A5 Antibody (1G5G9) - BSA Free

Western Blot: Apolipoprotein A5 Antibody (1G5G9)BSA Free [NB110-55454]

Western Blot: Apolipoprotein A5 Antibody (1G5G9)BSA Free [NB110-55454]

Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard.
Immunohistochemistry-Paraffin: Apolipoprotein A5 Antibody (1G5G9) - BSA Free [NB110-55454]

Immunohistochemistry-Paraffin: Apolipoprotein A5 Antibody (1G5G9) - BSA Free [NB110-55454]

Immunohistochemistry-Paraffin: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using 5 ug/ml conc. of Apolipoprotein A5 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Apolipoprotein A5 in hepatocytes was observed. Staining was performed by Histowiz.
Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) - BSA Free [NB110-55454]

Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) - BSA Free [NB110-55454]

Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Applications for Apolipoprotein A5 Antibody (1G5G9) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1 ug per million cells

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5 ug/ml
Application Notes
This Apolipoprotein A5 antibody is useful in ELISA, Immunohistochemistry paraffin embedded sections and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Apolipoprotein A5

Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).

Alternate Names

APOAV, apo-AV, APOA-V, Apolipoprotein A5, apolipoprotein A-V, FLJ97995, MGC126836, RAP3MGC126838, Regeneration-associated protein 3

Gene Symbol

APOA5

Additional Apolipoprotein A5 Products

Product Documents for Apolipoprotein A5 Antibody (1G5G9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein A5 Antibody (1G5G9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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