Apolipoprotein A5 Antibody (1G5G9)

Novus Biologicals, part of Bio-Techne | Catalog # NB400-139

Novus Biologicals, part of Bio-Techne
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NB400-139
NB400-139SS
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Key Product Details

Species Reactivity

Validated:

Human, Hamster

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G5G9

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Apolipoprotein A5 Antibodies »

Product Specifications

Immunogen

Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]

Reactivity Notes

Immunogen sequence has 83% identity to bovine, 79% identity to rat and 78% identity to mouse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein A5 Antibody (1G5G9)

Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139]

Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139]

Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Analysis of ApoA5 in HepG2 lysates.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) [NB400-139]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) [NB400-139]

Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Apolipoprotein A5 (1G5G9) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB400-139]

Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB400-139]

Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB400-139] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Applications for Apolipoprotein A5 Antibody (1G5G9)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
In Western blot a band at approximately 41 kDa is seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Apolipoprotein A5

Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).

Alternate Names

APOAV, apo-AV, APOA-V, Apolipoprotein A5, apolipoprotein A-V, FLJ97995, MGC126836, RAP3MGC126838, Regeneration-associated protein 3

Entrez Gene IDs

116519 (Human)

Gene Symbol

APOA5

UniProt

Q6Q788 (Human)

Additional Apolipoprotein A5 Products

Product Documents for Apolipoprotein A5 Antibody (1G5G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein A5 Antibody (1G5G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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