Apolipoprotein B/ApoB Antibody (APOB/4333)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07231

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # APOB/4333

Concentration

0.2 mg/ml

View all Apolipoprotein B/ApoB Antibodies »

Product Summary for Apolipoprotein B/ApoB Antibody (APOB/4333)

Immunogen

Human recombinant Apolipoprotein B/ApoB protein fragment (around aa 592-689) (exact sequence is proprietary) (Uniprot: P04114)

Localization

Cytoplasm, Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

512 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08230)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Apolipoprotein B/ApoB Antibody (APOB/4333)

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody (APOB/4333) [NBP3-07231]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody (APOB/4333) [NBP3-07231]

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody (APOB/4333) [NBP3-07231] - Formalin-fixed, paraffin-embedded human liver stained with Apolipoprotein B/ApoB Mouse Monoclonal Antibody (APOB/4333).
Protein Array: Apolipoprotein B/ApoB Antibody (APOB/4333) [NBP3-07231]

Protein Array: Apolipoprotein B/ApoB Antibody (APOB/4333) [NBP3-07231]

Protein Array: Apolipoprotein B/ApoB Antibody (APOB/4333) [NBP3-07231] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Apolipoprotein B/ApoB Antibody (APOB/4333).

Applications for Apolipoprotein B/ApoB Antibody (APOB/4333)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Apolipoprotein B/ApoB

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.

Alternate Names

APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4

Gene Symbol

APOB

UniProt

P04114

Additional Apolipoprotein B/ApoB Products

Product Documents for Apolipoprotein B/ApoB Antibody (APOB/4333)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Apolipoprotein B/ApoB Antibody (APOB/4333)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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