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Apolipoprotein B/ApoB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB200-527

Novus Biologicals, part of Bio-Techne
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NB200-527

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Mouse low density lipoprotein (LDL)

Reactivity Notes

Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved. Rat reactivity reported in scientific literature (PMID: 29964320).

Localization

Secreted

Specificity

Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apolipoprotein B/ApoB Antibody

Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527]

Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527]

Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.

Applications for Apolipoprotein B/ApoB Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID:31807238).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB200-527 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -70C. Avoid freeze-thaw cycles.

Background: Apolipoprotein B/ApoB

Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.

Alternate Names

APOB, ApoB-100, ApoB-48, FLDB, LDLCQ4

Entrez Gene IDs

338 (Human); 238055 (Mouse)

Gene Symbol

APOB

Additional Apolipoprotein B/ApoB Products

Product Documents for Apolipoprotein B/ApoB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein B/ApoB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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