Apolipoprotein CIII Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-610
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Bovine
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
produced from Apolipoprotein CIIII derived from human plasma (Uniprot: P02656)
Reactivity Notes
Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Bovine reactivity reported in scientific literature (PMID: 29852444).
Bovine reactivity reported in scientific literature (PMID: 29852444).
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Apolipoprotein CIII Antibody
Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NB600-610]
Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NB600-610] - Tissue: liver.Apolipoprotein CIII Antibody
Immunohistochemistry of Apolipoprotein CIII Antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Apolipoprotein C-III at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Apolipoprotein C-III as precipitated red signal with hematoxylin purple nuclear counterstain.Applications for Apolipoprotein CIII Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity.
Reviewed Applications
Read 1 review rated 4 using NB600-610 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: Apolipoprotein CIII
Alternate Names
APOC3, APOCIII, Apo-CIII, apoC-III, Apolipoprotein C3, apolipoprotein C-III, MGC150353
Entrez Gene IDs
345 (Human)
Gene Symbol
APOC3
UniProt
Additional Apolipoprotein CIII Products
Product Documents for Apolipoprotein CIII Antibody
Product Specific Notices for Apolipoprotein CIII Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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