Apolipoprotein CIII Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92734
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-99 of human Apolipoprotein C3 (NP_000031.1). MQPRVLLVVALLALLASARASEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDPEVRPTSAVAA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Apolipoprotein CIII Antibody - Azide and BSA Free
Western Blot: Apolipoprotein CIII AntibodyAzide and BSA Free [NBP2-92734]
Western Blot: Apolipoprotein CIII Antibody [NBP2-92734] - Analysis of extracts of various cell lines, using Apolipoprotein C3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.Immunocytochemistry/ Immunofluorescence: Apolipoprotein CIII Antibody - Azide and BSA Free [NBP2-92734]
Immunocytochemistry/Immunofluorescence: Apolipoprotein CIII Antibody [NBP2-92734] - Analysis of rat liver cells using Apolipoprotein C3 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody - Azide and BSA Free [NBP2-92734]
Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NBP2-92734] - Rat spleen using Apolipoprotein C3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Apolipoprotein CIII Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:100-1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Apolipoprotein CIII
Alternate Names
APOC3, APOCIII, Apo-CIII, apoC-III, Apolipoprotein C3, apolipoprotein C-III, MGC150353
Gene Symbol
APOC3
Additional Apolipoprotein CIII Products
Product Documents for Apolipoprotein CIII Antibody - Azide and BSA Free
Product Specific Notices for Apolipoprotein CIII Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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