Apolipoprotein E/ApoE Antibody (1H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22110

Novus Biologicals, part of Bio-Techne
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NBP2-22110
NBP2-22110SS
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H4

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Apolipoprotein E/ApoE Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ApoE expressed in E. coli. [UniProt# P02649]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein E/ApoE Antibody (1H4)

Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]

Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]

Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE mouse mAb with DAB staining.
Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of HepG2 cells using ApoE mouse mAb (green) and negative control (purple).
View 1 more images

Applications for Apolipoprotein E/ApoE Antibody (1H4)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein E/ApoE

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Tissue specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Alternate Names

APOE

Entrez Gene IDs

348 (Human)

Gene Symbol

APOE

UniProt

P02649

Additional Apolipoprotein E/ApoE Products

Product Documents for Apolipoprotein E/ApoE Antibody (1H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Apolipoprotein E/ApoE Antibody (1H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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