Apolipoprotein E/ApoE Antibody (PSH02-85)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32045
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH02-85
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human Apolipoprotein E/ApoE aa 101-150 / 317. (Uniprot: P02649)
Localization
Secreted, extracellular space, extracellular matrix.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Apolipoprotein E/ApoE Antibody (PSH02-85)
Western Blot: Apolipoprotein E/ApoE Antibody (PSH02-85) [NBP3-32045]
Western Blot: Apolipoprotein E/ApoE Antibody (PSH02-85) [NBP3-32045] - Western blot analysis of Apolipoprotein E/ApoE on different lysates with Rabbit anti-Apolipoprotein E/ApoE antibody (NBP3-32045) at 1/2,000 dilution.Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-APOE2 cell lysate
Lane 3: 293T-OE-APOE3 cell lysate
Lane 4: 293T-OE-APOE4 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 2 minutes 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32045) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Apolipoprotein E/ApoE Antibody (PSH02-85)
Application
Recommended Usage
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Apolipoprotein E/ApoE
Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.
The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.
Alternate Names
APOE
Gene Symbol
APOE
Additional Apolipoprotein E/ApoE Products
Product Documents for Apolipoprotein E/ApoE Antibody (PSH02-85)
Product Specific Notices for Apolipoprotein E/ApoE Antibody (PSH02-85)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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