I am interested in purchasing your apolipoprotein E antibody ID: NB110-60531. I would like to ask you which isotype control antibody you would recommend to go along with this antibody based on.

Our ApoE (WUE-4) antibody with catalogue number NB110-60531 is a mouse monoclonal of the isotype IgG1 kappa. The following product is an unconjugated mouse IgG1 kappa isotype control which should meet your requirements, however please note isotype control catalog # NBP1-43319 has not been validated in an identical range of applications to ApoE antibody NB110-60531.

I've ordered your ApoE Ab (Apolipoprotein E/ApoE Antibody (WUE-4); NB110-60531) to use for FACS, and one of your images reports that you just fixed with 4% PFA and then used 0.1% saponin. Is the saponin therefore sufficient/preferable for permeabilization (rather than using one of the commercially available fixation and permeabilization kits)? Presumably, the 0.1% saponin then needs to be added to the FACS buffer throughout until you the samples are run, to keep them permeabilized?

FLOW has worked with this protocol and was successful. So we can say it worked but will not say it is the only protocol you should use. We cannot comment on the commercial kit you are referring to since we have not tested directly but assume any protocol that correctly identifies the location of the target protein should be successful.

Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Name: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Brand: Novus Biologicals
SKU: NB110-60531
Rating: 5 (2 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60531

(2)

Citations (37)

Product Datasheet / COA / SDS / Protocols & Manuals

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NB110-60531AF350

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Alexa Fluor 405

NB110-60531AF405

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Alexa Fluor 488

NB110-60531AF488

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Alexa Fluor 532

NB110-60531AF532

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Alexa Fluor 594

NB110-60531AF594

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Alexa Fluor 647

NB110-60531AF647

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Alexa Fluor 700

NB110-60531AF700

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Alexa Fluor 750

NB110-60531AF750

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Allophycocyanin

NB110-60531APC

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Biotin

NB110-60531B

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CoraFluor 1

NB110-60531CL1

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DyLight 350

NB110-60531UV

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DyLight 405

NB110-60531V

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DyLight 488

NB110-60531G

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DyLight 550

NB110-60531R

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DyLight 594

NB110-60531DL594

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DyLight 650

NB110-60531C

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DyLight 680

NB110-60531FR

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DyLight 755

NB110-60531IR

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FITC

NB110-60531F

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HRP

NB110-60531H

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Janelia Fluor 525

NB110-60531JF525

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Janelia Fluor 549

NB110-60531JF549

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Janelia Fluor 585

NB110-60531JF585

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Janelia Fluor 635

NB110-60531JF635

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Janelia Fluor 646

NB110-60531JF646

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Janelia Fluor 669

NB110-60531JF669

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mFluor Violet 450 SE

NB110-60531MFV450

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mFluor Violet 500 SE

NB110-60531MFV500

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mFluor Violet 610 SE

NB110-60531MFV610

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NB110-60531PE

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PerCP

NB110-60531PCP

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat (Negative)

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # WUE-4

Format

BSA Free

Concentration

1.0 mg/ml

View all Apolipoprotein E/ApoE Antibodies »

Product Specifications

Immunogen

Purified human ApoE [UniProt# P02649]

Epitope

Residues 140-160 of purified human ApoE (LDLR receptor binding site).

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Localization

Secreted

Specificity

This antibody detects ApoE2, ApoE3 and ApoE4 (PMID 2466929).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4)BSA Free [NB110-60531]

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4)BSA Free [NB110-60531]

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mouse plasma. Image from confirmed customer review.

Immunocytochemistry/ Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE (WUE-4) [NB110-60531] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto latex beads. The EXOs were isolated from fasting PFP or PLT concentrate by differential UC and gravity size filtration (gray histograms: blocked beads incubated with antibody, empty histograms: EXO sample). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep24316), licensed under a CC-BY license.

Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody NB110-60531 [WUE-4] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NB110-60531AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE [WUE-4] Antibody NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

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Applications for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml

Application Notes

This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26564908). This antibody is CyTOF ready.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB110-60531 in the following applications:

ELISA (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein E/ApoE

ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.

Alternate Names

APOE

Gene Symbol

APOE

Additional Apolipoprotein E/ApoE Products

Product Documents for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download IHC-P Protocol

Download Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60531

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Apolipoprotein E/ApoE Antibodies »

Product Specifications

Immunogen

Epitope

Reactivity Notes

Localization

Specificity

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4)BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4)BSA Free [NB110-60531]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Applications for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

ELISA

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunoprecipitation

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Apolipoprotein E/ApoE

Alternate Names

Gene Symbol

Additional Apolipoprotein E/ApoE Products

Product Documents for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free