Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44434

Novus Biologicals, part of Bio-Techne
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NBP3-44434
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NBP2-14785
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0171

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Apolipoprotein L1/APOL1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O14791, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free

Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP3-44434]

Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP3-44434]

Staining of human duodenum shows strong positivity in plasma in blood vessels.
Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP3-44434]

Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP3-44434]

Staining of human colon shows moderate to strong positivity in plasma in blood vessels.
Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP3-44434]

Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP3-44434]

Staining of human skin shows no positivity in squamous epithelial cells as expected.
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Applications for Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein L1/APOL1

Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein L gene family encodes six highly homologous proteins designated apoL-I to -VI, which are associated with large high density type lipoproteins (HDL). The human apoL family maps to chromosome 22q12.1- 13.1 within a 127,000-bp region . apoL has been characterized as a pancreas specific, 383-amino acid, 42 kDa protein that contains a 12-amino acid secretory signal peptide. The apoL genes have TATA-less promoters and contain putative sterol regulatory elements, suggesting that transcription of these genes may be coordinated with that of the low density lipoprotein receptor and genes in pathways involving the synthesis of triglycerides and cholesterol. apoL homologs can undergo 10 fold changes in expression during atherosclerotic changes in vascular endothelial cells, which includes the inflammatory reaction of atherosclerotic lesions.

Long Name

Apolipoprotein L1

Alternate Names

APO-L, APO-L1, APO-LI, APOL, APOL1, FSGS4

Gene Symbol

APOL1

Additional Apolipoprotein L1/APOL1 Products

Product Documents for Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein L1 Antibody (CL0171) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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