Apolipoprotein L1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89033

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-89033
NBP1-89033-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Apolipoprotein L1/APOL1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SNFLSLAGNTYQLTRGIGKDIRALRRARANLQSVPHASASRPRVTEPISAESGEQVERVNEPSILEMSRGVKLTDVAPVSFFLVLDVVYLVYESKHLHEGAKSETAEELKKVAQELEEKLNILNN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apolipoprotein L1 Antibody

Western Blot: Apolipoprotein L1 Antibody [NBP1-89033]

Western Blot: Apolipoprotein L1 Antibody [NBP1-89033]

Western Blot: Apolipoprotein L1 Antibody [NBP1-89033] - Analysis in human cell lines A-431 and MCF-7 using anti-APOL1 antibody. Corresponding APOL1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein L1 Antibody [NBP1-89033]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein L1 Antibody [NBP1-89033]

Immunocytochemistry/Immunofluorescence: Apolipoprotein L1 Antibody [NBP1-89033] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis. The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins. Diabetes-related changes in the protein composition and the biomechanical properties of human retinal vascular basement membranes. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29284024/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP1-89033]

Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP1-89033]

Immunohistochemistry-Paraffin: Apolipoprotein L1 Antibody [NBP1-89033] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
View 4 more images

Applications for Apolipoprotein L1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID : 29284024).

Immunohistochemistry

1:2500 -1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Simple Western

1:30

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein L1/APOL1

Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein L gene family encodes six highly homologous proteins designated apoL-I to -VI, which are associated with large high density type lipoproteins (HDL). The human apoL family maps to chromosome 22q12.1- 13.1 within a 127,000-bp region . apoL has been characterized as a pancreas specific, 383-amino acid, 42 kDa protein that contains a 12-amino acid secretory signal peptide. The apoL genes have TATA-less promoters and contain putative sterol regulatory elements, suggesting that transcription of these genes may be coordinated with that of the low density lipoprotein receptor and genes in pathways involving the synthesis of triglycerides and cholesterol. apoL homologs can undergo 10 fold changes in expression during atherosclerotic changes in vascular endothelial cells, which includes the inflammatory reaction of atherosclerotic lesions.

Long Name

Apolipoprotein L1

Alternate Names

APO-L, APO-L1, APO-LI, APOL, APOL1, FSGS4

Gene Symbol

APOL1

Additional Apolipoprotein L1/APOL1 Products

Product Documents for Apolipoprotein L1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Apolipoprotein L1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.