Apolipoprotein M/ApoM Antibody (1F10)
Novus Biologicals, part of Bio-Techne | Catalog # H00055937-M01
Key Product Details
Species Reactivity
Human, Mouse
Applications
Dot Blot, ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1F10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
APOM (AAH20683, 23 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN
Reactivity Notes
Human. Other species not tested.
Specificity
APOM - apolipoprotein M
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Apolipoprotein M/ApoM Antibody (1F10)
![Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]](https://resources.bio-techne.com/images/products/Apolipoprotein-M-ApoM-Antibody-1F10-Western-Blot-H00055937-M01-img0006.jpg "Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]")
Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]
Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10.Lane 1: APOM transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.![Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]](https://resources.bio-techne.com/images/products/Apolipoprotein-M-ApoM-Antibody-1F10-Western-Blot-H00055937-M01-img0004.jpg "Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]")
Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01]
Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM over-expressed 293 cell line, cotransfected with APOM Validated Chimera RNAi ( Cat # H00055937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with APOM monoclonal antibody (M01), clone 1F10 (Cat # H00055937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Detection limit for recombinant GST tagged APOM is approximately 0.3ng/ml as a capture antibody.
Applications for Apolipoprotein M/ApoM Antibody (1F10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Apolipoprotein M/ApoM
Alternate Names
APOM, NG20, Protein G3a
Entrez Gene IDs
55937 (Human)
Gene Symbol
APOM
OMIM
606907 (Human)
UniProt
Additional Apolipoprotein M/ApoM Products
Product Documents for Apolipoprotein M/ApoM Antibody (1F10)
Product Specific Notices for Apolipoprotein M/ApoM Antibody (1F10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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