APOO Antibody (2F1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28870

Novus Biologicals, part of Bio-Techne
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NBP1-28870
NBP1-28870-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F1

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all APOO Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of ApoO expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Specific for ApoO.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APOO Antibody (2F1) - BSA Free

Western Blot: APOO Antibody (2F1)BSA Free [NBP1-28870]

Western Blot: APOO Antibody (2F1)BSA Free [NBP1-28870]

Western Blot: APOO Antibody (2F1) [NBP1-28870] - Analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.
Immunohistochemistry-Paraffin: APOO Antibody (2F1) - BSA Free [NBP1-28870]

Immunohistochemistry-Paraffin: APOO Antibody (2F1) - BSA Free [NBP1-28870]

Immunohistochemistry-Paraffin: APOO Antibody (2F1) [NBP1-28870] - Analysis of human Testis tissues using ApoO mouse mAb.

Applications for APOO Antibody (2F1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APOO

apolipoprotein O, also known as MYO25, FAM121B, MGC4825. Entrez Protein NP_077027. It is a chrondroitin-sulfate chain containing member of the apolipoprotein family and is an original glycoprotein up-regulated by diabetes in human Heart. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.

Alternate Names

apolipoprotein O, FAM121Bbrain my025, family with sequence similarity 121B, MGC4825, My025, MYO25, Protein FAM121B

Gene Symbol

APOO

Additional APOO Products

Product Documents for APOO Antibody (2F1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APOO Antibody (2F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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