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APOOL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89325

Novus Biologicals, part of Bio-Techne
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NBP1-89325
NBP1-89325-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAVKSLWTKSSKEESLPKPKEKTKLGSSSEIEVPAKTTHVLKHSVPLPTELSSEAKTKSESTSGATQFMPDPKLMDHGQSHPED

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23704930).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APOOL Antibody

Western Blot: APOOL Antibody [NBP1-89325]

Western Blot: APOOL Antibody [NBP1-89325]

Western Blot: APOOL Antibody [NBP1-89325] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: APOOL Antibody [NBP1-89325]

Immunocytochemistry/ Immunofluorescence: APOOL Antibody [NBP1-89325]

Immunocytochemistry/Immunofluorescence: APOOL Antibody [NBP1-89325] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: APOOL Antibody [NBP1-89325]

Immunohistochemistry-Paraffin: APOOL Antibody [NBP1-89325]

Immunohistochemistry-Paraffin: APOOL Antibody [NBP1-89325] - Staining of human rectum shows granular cytoplasmic positivity in glandular cells.

Applications for APOOL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APOOL

Alternate Names

AAIR8193, apolipoprotein O-like, chromosome X open reading frame 33, CXorf33, FAM121A, family with sequence similarity 121A, MGC129748, Protein FAM121A, UNQ8193

Entrez Gene IDs

139322 (Human)

Gene Symbol

APOOL

UniProt

Additional APOOL Products

Product Documents for APOOL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for APOOL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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