APP Antibody (6F10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26502
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6F10
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human APP [UniProt P05067]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for APP Antibody (6F10) - BSA Free
Western Blot: APP Antibody (6F10) [NBP3-26502] -
Western Blot: APP Antibody (6F10) [NBP3-26502] - Positive Western Blot detected in: Hela whole cell lysate, A375 whole cell lysate, HEK293 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate.All lanes: APP Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 87, 35, 77, 79, 79, 81, 83, 85, 86, 73, 85 kDa
Observed band size: 100 kDa
Immunocytochemistry/Immunofluorescence: APP Antibody (6F10) [NBP3-26502] -
Immunocytochemistry/Immunofluorescence: APP Antibody (6F10) [NBP3-26502] - Staining of Hela Cells with APP Antibody (6F10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for APP Antibody (6F10) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: APP
Long Name
Amyloid Precursor Protein
Alternate Names
Amyloid beta, beta Amyloid, Protease Nexin II
Gene Symbol
APP
Additional APP Products
Product Documents for APP Antibody (6F10) - BSA Free
Product Specific Notices for APP Antibody (6F10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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