APP Antibody (OTI7G9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70187

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7G9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all APP Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human APP (NP_000475) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APP Antibody (OTI7G9) - Azide and BSA Free

Western Blot: APP Antibody (OTI7G9)Azide and BSA Free [NBP2-70187]

Western Blot: APP Antibody (OTI7G9)Azide and BSA Free [NBP2-70187]

Western Blot: APP Antibody (OTI7G9) - Azide and BSA Free [NBP2-70187] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with a
Immunocytochemistry/ Immunofluorescence: APP Antibody (OTI7G9) - Azide and BSA Free [NBP2-70187]

Immunocytochemistry/ Immunofluorescence: APP Antibody (OTI7G9) - Azide and BSA Free [NBP2-70187]

Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) - Azide and BSA Free [NBP2-70187] - Staining of HepG2 cells using anti-Amyloid Precursor Protein mouse monoclonal antibody.
Immunohistochemistry: APP Antibody (OTI7G9) - Azide and BSA Free [NBP2-70187]

Immunohistochemistry: APP Antibody (OTI7G9) - Azide and BSA Free [NBP2-70187]

Immunohistochemistry: APP Antibody (OTI7G9) - Azide and BSA Free [NBP2-70187] - Staining of paraffin-embedded liver tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.
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Applications for APP Antibody (OTI7G9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APP

Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (A 4) protein has been found to be the principal constituent of senile plaques of AD's patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa A 4 protein. Two variants of A 4 can be detected; A 1-40 "short-tailed" and A 1-42 "long-tailed" (3-4).

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

Additional APP Products

Product Documents for APP Antibody (OTI7G9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for APP Antibody (OTI7G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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