APPL Antibody (OTI4B11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46536

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4B11

Concentration

1 mg/ml

View all APPL Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human APPL1 (NP_036228) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

79.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APPL Antibody (OTI4B11)

Western Blot: APPL Antibody (OTI4B11) [NBP2-46536]

Western Blot: APPL Antibody (OTI4B11) [NBP2-46536]

Western Blot: APPL Antibody (OTI4B11) [NBP2-46536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APPL1.
Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536]

Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536]

Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Human lymphoma tissue using anti-APPL1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536]

Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536]

Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APPL1 mouse monoclonal antibody.
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Applications for APPL Antibody (OTI4B11)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APPL

APPL is encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.

Long Name

Adapter Protein Containing PH Domain PTB Domain and Leucine Zipper Motif 1

Alternate Names

APPL1, DIP13A

Gene Symbol

APPL1

Additional APPL Products

Product Documents for APPL Antibody (OTI4B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APPL Antibody (OTI4B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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