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APPL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15434

Novus Biologicals, part of Bio-Techne
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NBP2-15434

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Porcine (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human APPL. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (89%), Zebrafish (86%), .

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APPL Antibody

Western Blot: APPL Antibody [NBP2-15434]

Western Blot: APPL Antibody [NBP2-15434]

Western Blot: APPL Antibody [NBP2-15434] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434]

Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434]

Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - Mouse muscle. APPL1 antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434]

Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434]

Immunohistochemistry-Paraffin: APPL Antibody [NBP2-15434] - AZ521 xenograft, using APPL1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for APPL Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: APPL

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq]

Long Name

Adapter Protein Containing PH Domain PTB Domain and Leucine Zipper Motif 1

Alternate Names

APPL1, DIP13A

Gene Symbol

APPL1

UniProt

Additional APPL Products

Product Documents for APPL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for APPL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.