Aquaporin 1/AQP1 Antibody (8V1O3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16354
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8V1O3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 170-269 of human Aquaporin-1 (Aquaporin 1/AQP1) (P29972). LAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aquaporin 1/AQP1 Antibody (8V1O3)
Western Blot: Aquaporin 1/AQP1 Antibody (8V1O3) [NBP3-16354]
Western Blot: Aquaporin 1/AQP1 Antibody (8V1O3) [NBP3-16354] - Western blot analysis of extracts of Mouse liver, using Aquaporin-1 (Aquaporin 1/AQP1) Rabbit mAb (NBP3-16354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Western Blot: Aquaporin 1/AQP1 Antibody (8V1O3) [NBP3-16354]
Western Blot: Aquaporin 1/AQP1 Antibody (8V1O3) [NBP3-16354] - Western blot analysis of extracts of various cell lines, using Aquaporin-1 (Aquaporin 1/AQP1) Rabbit mAb (NBP3-16354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (8V1O3) [NBP3-16354] -
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (8V1O3) [NBP3-16354] -Analysis of Aquaporin-1 (AQP1) in paraffin-embedded human breast cancer using Aquaporin-1 (AQP1) Rabbit mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for Aquaporin 1/AQP1 Antibody (8V1O3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aquaporin 1/AQP1
Alternate Names
AQP1, CHIP28, CO
Gene Symbol
AQP1
Additional Aquaporin 1/AQP1 Products
Product Documents for Aquaporin 1/AQP1 Antibody (8V1O3)
Product Specific Notices for Aquaporin 1/AQP1 Antibody (8V1O3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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