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Aquaporin 1/AQP1 Antibody (OTI2D10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03568

Novus Biologicals, part of Bio-Techne
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NBP2-03568
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D10

Concentration

0.47 mg/ml

Product Summary for Aquaporin 1/AQP1 Antibody (OTI2D10)

Immunogen

Full length human recombinant protein of human AQP1 (NP_932766) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aquaporin 1/AQP1 Antibody (OTI2D10)

Western Blot: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568]

Western Blot: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568]

Western Blot: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Analysis of extracts (10ug) from 3 different cell lines by using anti-AQP1 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568]

Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568]

Immunocytochemistry/Immunofluorescence: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aquaporin-1.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Staining of paraffin-embedded Human lymphoma tissue using anti-Aquaporin-1 mouse monoclonal antibody.

Applications for Aquaporin 1/AQP1 Antibody (OTI2D10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.47 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aquaporin 1/AQP1

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Alternate Names

AQP1, CHIP28, CO

Entrez Gene IDs

358 (Human)

Gene Symbol

AQP1

Additional Aquaporin 1/AQP1 Products

Product Documents for Aquaporin 1/AQP1 Antibody (OTI2D10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aquaporin 1/AQP1 Antibody (OTI2D10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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