Aquaporin-2 [p Ser261] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61100

Novus Biologicals, part of Bio-Techne
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NB100-61100
NB100-61100-0.02ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Canine

Predicted:

Bovine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Cytometric Bead Assay Standard, IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Aquaporin-2 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 conjugated to KLH. Accession # P34080

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25977473). Canine reactivity reported in scientific literature (PMID: 22778181).

Modification

p Ser261

Specificity

Specific for endogenous levels of the ~29 kDa Aquaporin-2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aquaporin-2 [p Ser261] Antibody

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100]

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100]

Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).

Applications for Aquaporin-2 [p Ser261] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:3000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

3 uL

Western Blot

1:1000
Application Notes
Immunohistochemistry as reported in the literature (Hoffert et al., 2007) Dot Blot was reported in scientific literature.

Reviewed Applications

Read 1 review rated 5 using NB100-61100 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aquaporin-2

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein aquaporins. Aquaporin 0, and Aquaporin 1 have been the foundation of the growing family of aquaporins. The lens specific Aquaporin 0 represents up to 80% of total lens membrane protein. Defects in Aquaporin 0 are a cause of autosomal dominant cataract. The lens opacity mutation is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin 0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C terminus are predicted to be cytoplasmic. Aquaporin 2 is located in the collecting tubule.

Alternate Names

ADH water channel, AQP-2, AQP-CD, aquaporin 2 (collecting duct), aquaporin-2, Aquaporin-CD, Collecting duct water channel protein, MGC34501, Water channel protein for renal collecting duct, water-channel aquaporin 2, WCH-CD

Entrez Gene IDs

359 (Human); 11827 (Mouse); 25386 (Rat)

Gene Symbol

AQP2

UniProt

P34080 (Rat)

Additional Aquaporin-2 Products

Product Documents for Aquaporin-2 [p Ser261] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Aquaporin-2 [p Ser261] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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