Aquaporin-2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-74682
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat, Porcine, Bovine, Camel
Predicted:
Bovine (100%), Porcine (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]
Reactivity Notes
Use in Rat reported in scientific literature (PMID:33634832).
Localization
Cell membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aquaporin-2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody - BSA Free [NB110-74682]
Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody - BSA Free [NB110-74682]
Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Western Blot: Aquaporin-2 AntibodyBSA Free [NB110-74682]
Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).Applications for Aquaporin-2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 ug/ml
Immunohistochemistry
1:100-1:200
Immunohistochemistry-Frozen
reported in scientific literature
Immunohistochemistry-Paraffin
1:100-1:200
Western Blot
1 - 2 ug/ml
Application Notes
Bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form in Western Blot.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: Aquaporin-2
Alternate Names
ADH water channel, AQP-2, AQP-CD, aquaporin 2 (collecting duct), aquaporin-2, Aquaporin-CD, Collecting duct water channel protein, MGC34501, Water channel protein for renal collecting duct, water-channel aquaporin 2, WCH-CD
Gene Symbol
AQP2
UniProt
Additional Aquaporin-2 Products
Product Documents for Aquaporin-2 Antibody - BSA Free
Product Specific Notices for Aquaporin-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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