Aquaporin-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-74682

Novus Biologicals, part of Bio-Techne
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NB110-74682
NB110-74682SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Porcine, Bovine, Camel

Predicted:

Bovine (100%), Porcine (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Aquaporin-2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33634832).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aquaporin-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody - BSA Free [NB110-74682]

Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody - BSA Free [NB110-74682]

Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody - BSA Free [NB110-74682]

Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody - BSA Free [NB110-74682]

Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: Aquaporin-2 AntibodyBSA Free [NB110-74682]

Western Blot: Aquaporin-2 AntibodyBSA Free [NB110-74682]

Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).
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Applications for Aquaporin-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 ug/ml

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Frozen

reported in scientific literature

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1 - 2 ug/ml
Application Notes
Bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form in Western Blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin-2

Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.

Alternate Names

ADH water channel, AQP-2, AQP-CD, aquaporin 2 (collecting duct), aquaporin-2, Aquaporin-CD, Collecting duct water channel protein, MGC34501, Water channel protein for renal collecting duct, water-channel aquaporin 2, WCH-CD

Entrez Gene IDs

359 (Human); 11827 (Mouse); 25386 (Rat)

Gene Symbol

AQP2

UniProt

P34080 (Rat)

Additional Aquaporin-2 Products

Product Documents for Aquaporin-2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aquaporin-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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