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Aquaporin-4 Antibody (AQP4/3324)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13952

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AQP4/3324

Concentration

0.2 mg/ml

Product Summary for Aquaporin-4 Antibody (AQP4/3324)

Immunogen

Human recombinant Aquaporin-4 protein fragment (around aa254-323) (exact sequence is proprietary) (Uniprot: P55087)

Localization

Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14104)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Aquaporin-4 Antibody (AQP4/3324)

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human brain stained with Aquaporin-4 antibody (AQP4/3324). Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952]

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Applications for Aquaporin-4 Antibody (AQP4/3324)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Aquaporin 4/AQP4

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Long Name

Aquaporin 4

Alternate Names

AQP-4, AQP4, MIWC, WCH4

Gene Symbol

AQP4

UniProt

Additional Aquaporin 4/AQP4 Products

Product Documents for Aquaporin-4 Antibody (AQP4/3324)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aquaporin-4 Antibody (AQP4/3324)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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